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    block this user Mahendra Kumar Trivedi

    Independent researcher

    Las Vegas Naveda
    Trivedi Global Inc.
    Trivedi Science Research Laboratory Pvt. Ltd

    An in Vitro Study of Biofield Energy Healing Based Herbomineral Formulation for Skin Protection

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    The current research work deals with the impact of the Consciousness Energy Healing Treatment (The Trivedi Effect®) based herbomineral test formulation and cell medium (DMEM) against various skin health parameters. The test formulation is the combination of minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, herbal (Centella asiatica) extract, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two equal parts, i.e. one part received the Biofield Energy Treatment by Eileen Mary Meagher and was labeled as the Biofield Treated (BT) samples, while other represented was defined as the untreated test (UT) samples. The MTT assay showed that the test formulation was found safe and nontoxic with greater than 75% cell viability. Cell proliferation assay using BrdU method showed an improved proliferation rate by 343.23%, 316.13%, and 512.75% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 17.5 µg/mL, compared with the untreated group. The collagen level was significantly increased by 15.70% and 13.52% at 2.5 and 0.625 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared with the untreated group. The level of elastin was increased by 47.0% and 64.1% at 2.5 and 1.25 µg/mL, respectively in the BT-DMEM + UT-Test formulation, group compared with the untreated group. Hyaluronic acid synthesis was increased by 4.37%, 41.47%, and 19.74% at 2.5, 1.25, and 0.625 µg/mL, respectively in the UT-DMEM + BT-Test formulation group with respect to the untreated group. Melanin synthesis was significantly inhibited by 8.83%, 5.78%, and 12.68% at 0.0125, 0.0625, and 0.125 µg/mL, respectively in the UT-DMEM + BT-Test formulation group compared with the untreated group. Anti-wrinkling effect showed an improved cell viability by 4.32% and 2.47% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + UT- Test formulation groups, respectively in HFF-1 cells compared with the untreated group. Wound healing activity using scratch assay exhibited improved a healing rate up to 6% in the HFF-1 and HaCaT cells lines in the Biofield Energy Treated Test formulation. In conclusion, results suggest that The Trivedi Effect®- Consciousness Energy Healing Treatment based test formulation and medium have the ability to improve the skin health and can be used as skincare products cosmetics for anti-ageing, anti-wrinkling action and for the treatment of many skin diseases such as Eczema, diaper rash, chickenpox, measles, warts, acne, hives, wrinkles, ringworm, Rosacea, psoriasis, seborrheic dermatitis, skin cancer, etc.

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    Description

    Title : An in Vitro Study of Biofield Energy Healing Based Herbomineral Formulation for Skin Protection
    Author(s) : Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Eileen Mary Meagher
    Abstract : The current research work deals with the impact of the Consciousness Energy Healing Treatment (The Trivedi Effect®) based herbomineral test formulation and cell medium (DMEM) against various skin health parameters. The test formulation is the combination of minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, herbal (Centella asiatica) extract, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two equal parts, i.e. one part received the Biofield Energy Treatment by Eileen Mary Meagher and was labeled as the Biofield Treated (BT) samples, while other represented was defined as the untreated test (UT) samples. The MTT assay showed that the test formulation was found safe and nontoxic with greater than 75% cell viability. Cell proliferation assay using BrdU method showed an improved proliferation rate by 343.23%, 316.13%, and 512.75% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 17.5 µg/mL, compared with the untreated group. The collagen level was significantly increased by 15.70% and 13.52% at 2.5 and 0.625 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared with the untreated group. The level of elastin was increased by 47.0% and 64.1% at 2.5 and 1.25 µg/mL, respectively in the BT-DMEM + UT-Test formulation, group compared with the untreated group. Hyaluronic acid synthesis was increased by 4.37%, 41.47%, and 19.74% at 2.5, 1.25, and 0.625 µg/mL, respectively in the UT-DMEM + BT-Test formulation group with respect to the untreated group. Melanin synthesis was significantly inhibited by 8.83%, 5.78%, and 12.68% at 0.0125, 0.0625, and 0.125 µg/mL, respectively in the UT-DMEM + BT-Test formulation group compared with the untreated group. Anti-wrinkling effect showed an improved cell viability by 4.32% and 2.47% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + UT- Test formulation groups, respectively in HFF-1 cells compared with the untreated group. Wound healing activity using scratch assay exhibited improved a healing rate up to 6% in the HFF-1 and HaCaT cells lines in the Biofield Energy Treated Test formulation. In conclusion, results suggest that The Trivedi Effect®- Consciousness Energy Healing Treatment based test formulation and medium have the ability to improve the skin health and can be used as skincare products cosmetics for anti-ageing, anti-wrinkling action and for the treatment of many skin diseases such as Eczema, diaper rash, chickenpox, measles, warts, acne, hives, wrinkles, ringworm, Rosacea, psoriasis, seborrheic dermatitis, skin cancer, etc.
    Keywords : Consciousness Energy Healing Treatment, Scratch Assay, Extracellular Matrix, Hyaluronic Acid, Tetrahydrocurcumin

    Subject : Skin Health
    Area : Health Sciences
    Language : English
    Year : 2017

    Affiliations Trivedi Global Inc.
    Journal : American Journal of Laboratory Medicine
    Volume : 2
    Issue : 2
    Publisher : Science Publishing Group
    Pages : 13-23
    Doi : 10.11648/j.ajlm.20170202.11
    Attribution Non-Commercial Share Alike

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    Mahendra's Peer Evaluation activity

    Downloads 32897
    Views 199
    Following... 21
    • Alejandro Engelmann, Independent researcher, Library, Swedish University of Agricultural Sciences, Uppsala, Sweden.
    • Selma Dorrestein, Student, Master Level, University of Amsterdam.
    • Francisco Herrera, Publisher, UNIVERSITY OF GRANADA.
    • Ralf Steinmetz, Professor, university.
    • Gregory Dudek, Professor, McGill University, School of Computer Science, Montreal, Canada.
    • Umberto Straccia, Senior Research Fellow, ISTI - CNR.
    • Sorin Cotofana, Associate Professor, Deft University of Technology, Faculty of Electrical Engineeting, Mathematics, and Computer Science. Computer Engineering, Delft, The Netherlands.
    • Stefan Trausan-Matu, Professor, Computer Science Department, Politehnica University of Bucharest, Research Institute for Artificial Intelligence.
    • Jean Quisquater, Professor, UCL Crypto Group.
    • Markus Jakobsson, Principal Research Fellow, PayPal, FatSkunk, Indiana University.
    • Michael Elad, Professor, Technion - Israel institute of Technology.
    • Andrew Lumsdaine, Professor, Indiana University.
    • Mikael Nilsson, Student, Ph.D. Level, Royal Institute of Technology, Stockholm, Sweden.
    • Emilie Combet, Lecturer, MVLS, University of Glasgow, Glasgow, Centre for Population and Health Sciences, Life-course Nutrition and Health.
    • Werner Muller, Professor, Faculty of Life Science, University of Manchester, Manchester.
    • Syam Mohan, Senior Research Fellow, Pharmacology, University of Malaya, Malaysia.
    • Ramy K Aziz, Lecturer, Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
    • Paweł K. Jędrzejko, Associate Professor, Department of American and Canadian Studies of the Institute of English Cultures and Literatures, University of Silesia in Katowice, Poland.
    • Nader Ale Ebrahim, Independent researcher, Research Support Unit, Centre of Research Services, Institute of Research Management and Monitoring (IPPP), University of Malaya, Malaysia.
    • Kelli Barr, Student, Ph.D. Level, Department of Philosophy and Religion Studies, University of North Texas, Denton, TX.
    • Pandelis Perakakis, Post Doctorate, Economics department, Universitet Jaume I, Castellon.

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