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    block this user Mahendra Kumar Trivedi

    Independent researcher

    Las Vegas Naveda
    Trivedi Global Inc.
    Trivedi Science Research Laboratory Pvt. Ltd

    Bacterial Identification Using 16S rDNA Gene Sequencing and Antibiogram Analysis on Biofield Treated Pseudomonas fluorescens

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    Biofield therapies have been reported to improve the quality of life as compared to other energy medicine. The aim of the study was to evaluate the impact of Mr. Trivedi’s biofield energy treatment on Pseudomonas fluorescens (P. fluorescens) for antimicrobial sensitivity, minimum inhibitory concentration (MIC), biochemical reactions, and biotype number. P. fluorescens cells were procured from MicroBioLogics Inc., USA in sealed packs bearing the American Type Culture Collection (ATCC 49838) number and divided in control and treated group. The effect was evaluated on day 10, and 159 after biofield treatment in lyophilized state. Further study was performed on day 5, 10, and 15 after retreatment on day 159 in revived state as per study design. All experimental parameters were studied using automated MicroScan Walk-Away® system. The 16S rDNA sequencing was carried out to correlate the phylogenetic relationship of P. fluorescens with other bacterial species after treatment. The results showed improved sensitivities and decreased MIC value of aztreonam, cefepime, moxifloxacin, and tetracycline in revived and lyophilized treated sample with respect to the control. Arginine, cetrimide, kanamycin, and glucose showed altered biochemical reactions after biofield treatment with respect to control. Biotype numbers were altered along with species in lyophilized as well as in revived group. Based on nucleotides homology and phylogenetic analysis using 16S rDNA gene sequencing, treated sample was detected to be Pseudomonas entomophila (GenBank Accession Number: AY907566) with 96% identity of gene sequencing data, which was nearest homolog species to P. fluorescens (Accession No. EF672049). These findings suggest that Mr. Trivedi’s unique biofield treatment has the capability to alter changes in pathogenic P. fluorescens even in the lyophilized storage condition and can be used to modify the sensitivity of microbes against antimicrobials.

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    Description

    Title : Bacterial Identification Using 16S rDNA Gene Sequencing and Antibiogram Analysis on Biofield Treated Pseudomonas fluorescens
    Author(s) : Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak
    Abstract : Biofield therapies have been reported to improve the quality of life as compared to other energy medicine. The aim of the study was to evaluate the impact of Mr. Trivedi’s biofield energy treatment on Pseudomonas fluorescens (P. fluorescens) for antimicrobial sensitivity, minimum inhibitory concentration (MIC), biochemical reactions, and biotype number. P. fluorescens cells were procured from MicroBioLogics Inc., USA in sealed packs bearing the American Type Culture Collection (ATCC 49838) number and divided in control and treated group. The effect was evaluated on day 10, and 159 after biofield treatment in lyophilized state. Further study was performed on day 5, 10, and 15 after retreatment on day 159 in revived state as per study design. All experimental parameters were studied using automated MicroScan Walk-Away® system. The 16S rDNA sequencing was carried out to correlate the phylogenetic relationship of P. fluorescens with other bacterial species after treatment. The results showed improved sensitivities and decreased MIC value of aztreonam, cefepime, moxifloxacin, and tetracycline in revived and lyophilized treated sample with respect to the control. Arginine, cetrimide, kanamycin, and glucose showed altered biochemical reactions after biofield treatment with respect to control. Biotype numbers were altered along with species in lyophilized as well as in revived group. Based on nucleotides homology and phylogenetic analysis using 16S rDNA gene sequencing, treated sample was detected to be Pseudomonas entomophila (GenBank Accession Number: AY907566) with 96% identity of gene sequencing data, which was nearest homolog species to P. fluorescens (Accession No. EF672049). These findings suggest that Mr. Trivedi’s unique biofield treatment has the capability to alter changes in pathogenic P. fluorescens even in the lyophilized storage condition and can be used to modify the sensitivity of microbes against antimicrobials.
    Keywords : Pseudomonas Fluorescens, Biofield Treatment, Lyophilized State, Antimicrobial Susceptibility, Biochemical Reactions, Biotype, 16S rDNA Sequencing

    Subject : Microbiology
    Area : Health Sciences
    Language : English
    Year : 2015

    Affiliations Trivedi Global Inc.
    Trivedi Science Research Laboratory Pvt. Ltd
    Journal : Clinical & Medical Biochemistry: Open Access
    Volume : 1
    Issue : 1
    Publisher : Omics Publishing Group
    Doi : 10.4172/2471- 2663.1000101
    Attribution Non-Commercial Share Alike

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    Mahendra's Peer Evaluation activity

    Downloads 38236
    Views 200
    Following... 21
    • Alejandro Engelmann, Independent researcher, Library, Swedish University of Agricultural Sciences, Uppsala, Sweden.
    • Selma Dorrestein, Student, Master Level, University of Amsterdam.
    • Francisco Herrera, Publisher, UNIVERSITY OF GRANADA.
    • Ralf Steinmetz, Professor, university.
    • Gregory Dudek, Professor, McGill University, School of Computer Science, Montreal, Canada.
    • Umberto Straccia, Senior Research Fellow, ISTI - CNR.
    • Sorin Cotofana, Associate Professor, Deft University of Technology, Faculty of Electrical Engineeting, Mathematics, and Computer Science. Computer Engineering, Delft, The Netherlands.
    • Stefan Trausan-Matu, Professor, Computer Science Department, Politehnica University of Bucharest, Research Institute for Artificial Intelligence.
    • Jean Quisquater, Professor, UCL Crypto Group.
    • Markus Jakobsson, Principal Research Fellow, PayPal, FatSkunk, Indiana University.
    • Michael Elad, Professor, Technion - Israel institute of Technology.
    • Andrew Lumsdaine, Professor, Indiana University.
    • Mikael Nilsson, Student, Ph.D. Level, Royal Institute of Technology, Stockholm, Sweden.
    • Emilie Combet, Lecturer, MVLS, University of Glasgow, Glasgow, Centre for Population and Health Sciences, Life-course Nutrition and Health.
    • Werner Muller, Professor, Faculty of Life Science, University of Manchester, Manchester.
    • Syam Mohan, Senior Research Fellow, Pharmacology, University of Malaya, Malaysia.
    • Ramy K Aziz, Lecturer, Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
    • Paweł K. Jędrzejko, Associate Professor, Department of American and Canadian Studies of the Institute of English Cultures and Literatures, University of Silesia in Katowice, Poland.
    • Nader Ale Ebrahim, Independent researcher, Research Support Unit, Centre of Research Services, Institute of Research Management and Monitoring (IPPP), University of Malaya, Malaysia.
    • Kelli Barr, Student, Ph.D. Level, Department of Philosophy and Religion Studies, University of North Texas, Denton, TX.
    • Pandelis Perakakis, Post Doctorate, Economics department, Universitet Jaume I, Castellon.

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